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Axis break in SigmaPlot?
Does anyone know how to create axis breaks in SigmaPlot 12.5? I would like the scale before the break to range from 0-140 and after the break to range from 1000-5000. The 0-140 range can be in increments of 20, the 1000-5000 range in increments of 1000. I've tried to do this but the y axis turns out looking all jumbled with so many numbers. Also, the break seems to disappear. Any suggestions?
I need help with custom non-linear regression in SigmaPlot 12.0
Hi there,submitted by Broxios to labrats
I am trying to measure the binding constant of a lipocalin to Retinol by means of fluorescence quenching for my diploma thesis. Our lab doesn't have a spectrofluorometer with stirrable cuvettes. However we do own a microplate reader which is capable of reading fluorescence intensity (Tecan Infinite M200) so I had to do my "titration" in a microplate. When I plot my data the graph looks just like a typical fluorescence quenching plot.
I found this paper by Norris et al. (source is at the end of the post) where they derived an equation that would fit the type of binding in my experiment. But i can't get this equation to work for fitting in SigmaPlot. I added the custom equation as
f=a-((1+(b+c)*d-sqrt((b+c)^2*d^2+2*(b+c)*d+1))/(2*b*d))*(a-e) fit f to yand variables are
c=col(1) y=col(2)But I don't know what to write for Initial Parameters or Constraints. Norris et al. only said to constraint the parameter for Protein concentration (it's the b in my case).
I would be really grateful for any tips. I wanted to use SigmaPlot because we have it on the computers on campus. If somebody knows a better program for this which is free, I would be open for any suggestions.
Norris et al.: Norris, Andrew W.; Cheng, Lin; Giguère, Vincent; Rosenberger, Michael; Li, Ellen (1994): Measurement of subnanomolar retinoic acid binding affinities for cellular retinoic acid binding proteins by fluorometric titration. In: Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 1209 (1), S. 10–18. DOI: 10.1016/0167-4838(94)90130-9.